Genomics?assisted breeding for successful development of multiple?stress?tolerant, climate?smart rice for southern and southeastern Asia

Rice (Oryza sativa L.) in rainfed marginal environments is prone to multiple abiotic and biotic stresses, which can occur combination a single cropping season adversely affect rice growth yield. The present study was undertaken develop high-yielding, climate-resilient that provide tolerance stresses. An assembled first-crossing scheme employed transfer 15 quantitative trait loci (QTL) genes—qDTY1.1, qDTY2.1, qDTY3.1, qDTY12.1 (drought), Sub1 (submergence), Gm4 (gall midge), Pi9, Pita2 (blast), Bph3, Bph17 (brown plant hoppers), Xa4, xa5, xa13, Xa21, Xa23 (bacterial leaf blight)—from eight different parents using genomics-assisted breeding. A funnel mating design assemble all the targeted QTL genes into high-yielding breeding line IR 91648-B-1-B-3-1. Gene-based linked markers were used each generation from intercrossing F6 for tracking presence of desirable alleles genes. Single-plant selections performed F2 onwards select recombinants possessing interest with suitable phenotypes. Phenotyping 95 homozygous lines carrying six 10 nonstress, reproductive-stage (RS) drought, blast, bacterial blight (BLB), gall midge (GM), grain quality parameters such as chalkiness, amylose content (AC), gelatinization temperature (GT), head recovery (HRR). Finally, 56 F7 found promising multiple-location evaluation yield (GY) other traits. These multiple-stress-tolerant desired profiling be varietal release southern southeastern Asia through national systems.